The yeast oligosaccharyltransferase complex can be replaced by STT3 from Leishmania major
نویسندگان
چکیده
منابع مشابه
All in one: Leishmania major STT3 proteins substitute for the whole oligosaccharyltransferase complex in Saccharomyces cerevisiae.
The transfer of lipid-linked oligosaccharide to asparagine residues of polypeptide chains is catalyzed by oligosaccharyltransferase (OTase). In most eukaryotes, OTase is a hetero-oligomeric complex composed of eight different proteins, in which the STT3 component is believed to be the catalytic subunit. In the parasitic protozoa Leishmania major, four STT3 paralogues, but no homologues to the o...
متن کاملPhotocross-linking of nascent chains to the STT3 subunit of the oligosaccharyltransferase complex
In eukaryotic cells, polypeptides are N glycosylated after passing through the membrane of the ER into the ER lumen. This modification is effected cotranslationally by the multimeric oligosaccharyltransferase (OST) enzyme. Here, we report the first cross-linking of an OST subunit to a nascent chain that is undergoing translocation through, or integration into, the ER membrane. A photoreactive p...
متن کاملCan Anaphoric Definite Descriptions be Replaced by Pronouns?
Abstract We investigate the hypothesis that a pronoun is used in discourse, when its antecedent is in the focus of the discourse. We create a corpus of ‘replaced anaphoric definite descriptions’, where occurrences of definite descriptions are replaced with a corresponding pronoun. We use the Lappin and Leass (1994) anaphora resolution algorithm on the new corpus, and obtain a much lower perform...
متن کاملAn essential type I nitroreductase from Leishmania major can be used to activate leishmanicidal prodrugs.
Nitroaromatic prodrugs are used to treat a range of microbial infections with selectivity achieved by specific activation reactions. For trypanosomatid parasites, this is mediated by type I nitroreductases. Here, we demonstrate that the causative agent of leishmaniasis, Leishmania major, expresses an FMN-containing nitroreductase (LmNTR) that metabolizes a wide range of substrates, and based on...
متن کاملCan Intravenous Urography be Replaced by CT Urography? Our Experience in the Evaluation for Hematuria
This study was conducted for comparing the diagnostic rate of CT and IVU for hematuria and establishing a proper diagnostic protocol During the year of 2004, total 32 patients presented with hematuria received both IVU and CT examination. The CT examination included three phase axial scan: noncontrast (phase I), 90 seconds (phase II) and 180 seconds (phase III) after contrast administration and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Glycobiology
سال: 2008
ISSN: 0959-6658,1460-2423
DOI: 10.1093/glycob/cwn118